Research

Chaperone-mediated Proteasome Assembly

1. How do chaperones mediate "nucleotide-dependentÌýswitches"Ìýto complete proteasome holoenzymes properly?

Chaperone-mediated proteasome assembly is an incredibly sophisticated process, which initiates with 2 chaperones (Nas6, Rpn14; red, green) and their cognate ATPase subunits, Rpt3 and Rpt6 (orange). We identified nucleotide-dependent activities of chaperones, using Nas6 (Gankyrin, onco-protein in humans). Nas6 alternates its steric hindrance against lid or CP, depending nucleotide-state of the bound ATPase (orange), untilÌýa heterohexameric ATPase ring assembly (orange) is complete. Currently, we are investigating whether and how Nas6 cooperatively actsÌýwith itsÌýneighboring chaperone, Rpn14 (green), in order to properly "switch" to late-stage intermediates, and to the final step of completing proteasome holoenzyme.Ìý

In early-stage assembly intermediate (left), Nas6 (red) obstructs lid (blue), thereby allowing the ATPase subunits (orange) to assemble into a heterohexameric ring. In late intermediate (middle), the fully-formed heterohexameric ATPase ring initiates ATP hydrolysis, relieving Nas6 steric clash against the lid, allowing lid-base assembly. At this stage, Nas6 together with Rpn14 (green) and Hsm3 (yellow) hinder CP binding. Upon completion of proteasome holoenzyme (right), chaperones are evicted.

Ìý

Ubiquitin-dependent Switch

2. How does a "ubiquitin-dependent switch" prevent formation of faulty proteasome holoenzymes?

In panel A, Rpt5 C-domain (orange) is shown with Not4-targeted ubiquitination sites highlighted in yellow. These sites are normally obstructed by Nas2-C domain (dark pink). In panel B, the same Rpt5-C domain with ubiquitination sites are obstructed by Hsm3 (green), due to its proximity to Rpt5 within the heterohexameric Rpt ring.
We discovered that Not4, an E3 ubiquitin ligase, selectively recognizes a heterohexameric ATPase ring that assembles without a chaperone, by ubiquitinatingÌýRpt5 (orange). Normally, ubiquitination target sites in Rpt5 are obstructed by 2 chaperones: Nas2 (pink, A) or Hsm3 (green, B).ÌýThis discovery inspired several follow-up investigations.

First, we are investigatingÌýhow Nas2 and Hsm3 act on this penultimate step of a heterohexameric ATPase ring assembly, using a heterologous E.coli expression system in vitro, since we can readily manipulate start and stop point of assembly. Second, we are investigatingÌýan additional mechanism of Not4 on chaperone-mediated proteasome assembly, since Not4 also acts as a part of a highly conserved transcriptional and translational regulator, Ccr4-Not complex. Third, we are investigating the fate of a heterohexameric ATPase ring, which is ubiquitinated by Not4, and how this ubiquitination can beÌýreversibly removed to allow the corrected assembly intermediates to proceed to complete proteasome holoenzyme.ÌýÌý

Ìý

Live-cell confocal images are shown. In nutrient-rich conditions (a, b), proteasomes concentrate in the nucleus. By contrast, during nutritional stress (c, d), only the normal proteasome dramatically re-localize to cytoplasmic storage granules (c, arrowheads, magenta) whereas misassembled proteaosmes (d) do not.
Proteasome Storage Granule

3. How do cells survive nutriotional stress by "remodeling" proteasomes for storageÌýagainstÌýautophagy?

When nutrients are abundant, proteasomes are enriched in the nucleus where they degrade key regulators of fundamental processes, such as transcription factors and cell cycle regulators. By contrast, when nutrients are scarce, proteasomes dramatically remodel, exit the nucleus, and store in cytoplasmic "proteasome storage granule"Ìýfor protection against autophagy. Only the properly assembled proteasomes can be stored, andÌýmisassembled proteasomes cannot. We aim to identify how de novo proteasome assembly via chaperones influencesÌýthe fate of proteasomesÌýduring nutriontional stress, and how cancer cells overexpressingÌýa knownÌýonco-protein, Gankyrin, influence proteasome storage and protection against autophagy for their survival, as they encounter nutriotional fluctuations.Ìý

Ìý