Super-Resolution

THOR - Nikon A1 laser scanning, structured illumination and TIRF

Lasers & Filters

Lasers:

Lauch: LUNV6, high-output.

Excitation at: 405, 445, 488, 515, 561, 647 (nm)

Emission (Chroma) and molecules imaged:

  • Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
  • Emission filter: 467.5-502.5 (nm): CFP, cerulean, etc.Ìý
  • Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc.Ìý
  • Emission filter: 521.5-554.5 (nm): YFP, Venus, etc.Ìý
  • Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.Ìý
  • Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc.Ìý
  • DIC imaging possible (laser scanning confocal only)

Filters for widefield:

DAPI, GFP, RFP, Brightfield

N-SIM certified objectives:

  • 100x 1.49NA OIL SR Apo TIRF WD 0.12 (mm)

  • 60X 1.27NA Water SR Plan Apo WD 0.17 (mm)

Standard objectives:

  • 4x 0.2NA Plan ApoÌýλ WD 20 (mm)
  • 10x 0.45NA Plan Apo λ WD 4 (mm)
  • 20x 0.75NA Plan Apo VC λ WD 1 (mm)
  • 40x 0.6NA S Plan Fluor ELWD 3.6-2.8 (mm)
  • 40x 0.95NA Plan Apo λ WD 0.25-0.17 (mm)

Available upon request:Ìý

  • 60x 1.40NA Plan Apo OIL VC λ WD 0.13 (mm)

Laser Scanning Confocal:

  • 2 x GaAsP high-sensitivty PMTs (CFP, GFP, YFP, RFP)
  • 2 x multi-alkali PMTs (DAPI, far-red)

N-SIM:

Ìý512 X 512 16 bit

Ìý

Ìý

  • TOKAI-HIT Cell Incubator 1 x 35 (mm) dish or chamber side (coverslip bottom)
  • Motorized X, Y, Z and separate Mad City Labs Piezo Z
  • Multi-point, time-lapse, optical sectioning, large-area montage with stitching
  • Increase power over imaging depth
  • Ìý5.21.03; 64 bit

THOR - Nikon Structured Illumination Super-resolution and A1 Laser Scanning Confocal Microscope (Left View)

THOR - Nikon Structured Illumination Super-resolution and A1 Laser Scanning Confocal Microscope (Front View)

THOR - Nikon Structured Illumination Super-resolution and A1 Laser Scanning Confocal Microscope (Right View)

THOR - Raw Data of 3-D SIM Image Showing 5 Phase Translations Per Grid Rotation and 3 Rotational Angles.

THOR - Nikon Raw Data of 3-D SIM Image Showing Interference Fringes (Images provided by the Orth Lab).